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	Provedor de dados BJMBR (2) Autor Langella,P. (2) Le Loir,Y. (2) Azevedo,V.A. (1) Correa Junior,A. (1) Freitas,D.A. (1) Gautier,M. (1) Leclerc,S. (1) Miyoshi,A. (1) Oliveira,S.C. (1) Rodrigues,L. (1) Sommer,P.S.M. (1) Mais... Palavra-chave Lactococcus lactis (2) Antifungal protein (1) Bovine coronavirus (1) Epitope (1) Propeptide (1) Protein secretion (1) Secretion (1) Staphylococcal nuclease (1) Streptomyces tendae antifungal protein 1 (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2005 (1) 1999 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Heterologous protein secretion in Lactococcus lactis: a novel antigen delivery system BJMBR Langella,P.; Le Loir,Y.. Lactic acid bacteria (LAB) are Gram-positive bacteria and are generally regarded as safe (GRAS) organisms. Therefore, LAB could be used for heterologous protein secretion and they are good potential candidates as antigen delivery vehicles. To develop such live vaccines, a better control of protein secretion is required. We developed an efficient secretion system in the model LAB, Lactococcus lactis. Staphylococcal nuclease (Nuc) was used as the reporter protein. We first observed that the quantity of secreted Nuc correlated with the copy number of the cloning vector. The nuc gene was cloned on a high-copy number cloning vector and no perturbation of the metabolism of the secreting strain was observed. Replacement of nuc native promoter by a strong... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protein secretion; Staphylococcal nuclease; Lactococcus lactis; Propeptide; Bovine coronavirus; Epitope. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200007 Secretion of Streptomyces tendae antifungal protein 1 by Lactococcus lactis BJMBR Freitas,D.A.; Leclerc,S.; Miyoshi,A.; Oliveira,S.C.; Sommer,P.S.M.; Rodrigues,L.; Correa Junior,A.; Gautier,M.; Langella,P.; Azevedo,V.A.; Le Loir,Y.. Lactococcus lactis, the model lactic acid bacterium, is a good candidate for heterologous protein production in both foodstuffs and the digestive tract. We attempted to produce Streptomyces tendae antifungal protein 1 (Afp1) in L. lactis with the objective of constructing a strain able to limit fungal growth. Since Afp1 activity requires disulfide bond (DSB) formation and since intracellular redox conditions are reportedly unfavorable for DSB formation in prokaryotes, Afp1 was produced as a secreted form. An inducible expression-secretion system was used to drive Afp1 secretion by L. lactis; Afp1 was fused or not with LEISSTCDA, a synthetic propeptide (LEISS) that has been described to be a secretion enhancer. Production of Afp1 alone was not achieved, but... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactococcus lactis; Antifungal protein; Secretion; Streptomyces tendae antifungal protein 1. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100004 Registros recuperados: 2 Primeira ... 1 ... Última

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